Shimadzu Nexera UHPLC Operating Procedures: Complete Analysis Protocol Guide

Ultra-High Performance Liquid Chromatography (UHPLC) represents the evolution of traditional HPLC, offering dramatically improved resolution, sensitivity, and speed through the use of sub-2 μm particle columns operating at pressures up to 130 MPa (approximately 19,000 psi). The Shimadzu Nexera series stands at the forefront of UHPLC technology, combining cutting-edge hardware with Analytical Intelligence features including AI-driven diagnostics, automated method development, and IoT-based laboratory management systems. The Nexera family encompasses multiple models designed for diverse analytical requirements: the Nexera X3 flagship system handles extreme pressure applications up to 130 MPa for the most demanding separations; the Nexera XS provides versatile 105 MPa capability with optional inert flow paths for biomolecule analysis; the Nexera XR offers 70 MPa performance for standard UHPLC and advanced HPLC methods; and the Nexera lite series delivers reliable 44 MPa operation for routine applications. Each model incorporates Shimadzu's proprietary technologies including Nexflow for minimized extra-column dispersion, FlowPilot for intelligent flow control protecting columns from pressure shock, and i-PDeA II for intelligent peak deconvolution enabling accurate quantitation of co-eluted compounds. With over 15,550 scientific citations and consistently high user ratings on research platforms, the Nexera series has become the instrument of choice for pharmaceutical development, environmental monitoring, food safety testing, and cutting-edge research applications. This comprehensive guide provides detailed operating procedures ensuring optimal performance, regulatory compliance, and laboratory safety across all Nexera models, with specific attention to the unique capabilities and requirements of each system configuration.